Apex™ Taq Master Mixes are ready-to-use 2.0x reaction mixes. They contain Apex Taq DNA Polymerase, an Ammonium based Buffer System, Apex ultra pure dNTPs (>99%) and Magnesium Chloride. Simply add primers, template, and water to successfully carry out primer extension and other molecular biology applications. Master Mixes offer convenience and reproducibility as reactions can be set up with less pipetting steps. The number of reactions is based on using 25μl of the master mix for a final reaction volume of 50μl. If desired, the reaction size may be scaled down to a final volume of 20 or 25μl by using 10μl or 12.5μl of the master mix respectively.
Apex Taq DNA Polymerase possesses 5'-3' DNA polymerase activity and a double strand 5'-3' exonuclease activity. It leaves an 'A' overhang which makes it ideal for TA cloning. The robust nature of this enzyme means that Apex Taq can be used with little optimization.