The?Mix & Go E. coli?Transformation Kit and?Mix & Go E. coli?Transformation Buffer Set are convenient methods for the preparation of competent?E. coli?cells for simple and highly efficient DNA transformation. The?Mix & Go?method completely eliminates the requirement for heat shocking and related procedures. Instead,?Mix & Go?bacterial transformation can be performed by adding DNA to?Mix & Go?competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 10^8-10^9?transformants/ug plasmid DNA with most?E. colistrains.
Uniquely formulated reagents make it easy to generate Mix & Go competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go E. coli Transformation Kit includes all buffers and ZymoBroth™ medium to generate 20 ml of Mix & Go competent cells. The Mix & Go E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go competent cells, and the medium (broth) is supplied by the user.