PCRBIO Hot Start VeriFi polymerase is a high fidelity enzyme derived from Pfu DNA polymerase for it’s 3’-5’ exonuclease (proofreading) activity. This 2X hot start mix is able to amplify longer and more difficult targets such as eukaryotic genomic templates up to 17.5kb, and longer for simpler DNA templates. PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’-3’ polymerase activity of the enzyme at ambient temperatures. This unique hot start molecule prevents primer dimer formation and nonspecific amplification to maximize the sensitivity and specificity of your PCR. The AptaLock™ technology makes this polymerase highly suitable for multiplexing and allows for reactions to be set up at room temperature.
The enhanced accuracy of PCRBIO Hot Start VeriFi™ Mix gives extremely low error rates and fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideal for applications where superior accuracy is required, such as cloning, site-directed mutagenesis and sequencing. This advanced 2X ready mix contains an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes regardless of GC or AT content.