Apex qPCR 2X Master Mixes for Probe are single-tube 2x reagents including all components necessary to perform probe based (TaqMan) real-time DNA amplification. You just need to add your primers, probe and DNA. Apex qPCR 2X Master Mixes for probe are available with high, low or without ROX for optimal performance on most of the commonly used real-time PCR instruments. Apex qPCR 2X Master Mixes promote high specificity and low background by using Apex Hot Start DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The unopened product is stable at -20 °C for 2 years. Store the qPCR 2X Master Mix at +4 °C after thawing. Once thawed, full activity is guaranteed for 3 months.
Applications include genomic and cDNA, absolute and relative quantitation, presence/absence experiments, SNP analysis, genotyping, multiplexing, and pathogen detection. Detection limit: Approximately 2 copies (~0.007 ng of human gDNA, correlating to 1 diploid genome, with 2 gene copies per diploid genome). Quantification limit: Approximately 24 copies (0.08 ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)