The clone 3G8, a mouse monoclonal antibody, binds to the 50-65 kDa transmembrane protein of IgG Fc?RIII known as CD16, a surface antigen commonly associated with human NK cells. In addition to surface expression on NK cells, CD16 is expressed on activated monocytes, macrophages, neutrophils, and placental trophoblasts in humans. 3G8 binds either with the aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell mediated cytotoxicity (ADCC). Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
iFluor™ fluorophores are optimized for strong fluorescence, high photostability and pH independence on proteins. They are avaialble to span the full UV-visible-IR spectrum. iFluor™647 is a great alternative to Alexa Fluor? 647, eFluor? 660. Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5μg (unconjugated, Biotin) or 5μl (conjugated) of these products are sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Non-human primate cross-reactivity has been validated using the APC conjugated clone 3G8 product. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].