Clone M5E2 reacts with a nonfunctional domain of human CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)- anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, the CD14 reacts with monocytes, interfollicular macrophages, reticular dendritic cells and some Langerhans cells. The binding of the CD14 does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5μg (unconjugated, Biotin) or 5μl (conjugated) of these products are sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Non-human primate cross-reactivity has been validated using Caprico’s FITC conjugated M5E2 product. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].