The ZR Plasmid Miniprep™-Classic is designed for efficient isolation of plasmid DNA from E. Coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast-Spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. The innovative Zymo-Spin™ llN columns yield endotoxin-free plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Yield Up to 25 μg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. Purity: Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A(260/280) greater than 1.8. Extra Zymo-Spin Columns: Uncapped: Cat # 11-502N & 11-503N; Capped: Cat # 11-502C & 11-503C