The Zyppy™-96 Plasmid Miniprep is the fastest and simplest high-throughput method available for efficient isolation of plasmid DNA from E. coli. The kit features a modified, Pellet-Free alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization/Clearing Buffer. The remaining DNA purification steps are straightforward and simple. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.
Up to 10 μg per preparation, depending on the plasmid copy number, culture growth conditions, and the strain of E. Coli processed.