The ZR BAC DNA Miniprep Kit is for the efficient isolation of BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
Up to 10 μg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed,and culture growth conditions. The typical yield is 1 μg per 3 ml of culture.