The ZymoPURE™ Plasmid Gigaprep kit features a spin column-based method for the purification of up to 10 mg of high-quality plasmid DNA in less than 50 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 2.5 μg/μl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ Plasmid Gigaprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.