The YeaStar™ RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 μg of total RNA from 1-1.5 ml of cultured cells using innovative Fast-Spin column technology.
Typically, RNA is eluted into 60 μl RNase-free water. The provided columns have an RNA binding capacity of 25 μg.