Prometheus™ Ni-NTA Agarose resin is is a high-capacity, high-performance nickel-IMAC resin specifically designed for affinity purification of His-tagged (6XHis) fusion proteins. It can be used under native and denaturing conditions to recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells.?Prometheus™ Ni-NTA Agarose uses nitrilotriacetic acid (NTA - a tetradenate chelating ligand) in a cross-linked 6% agarose matrix and is loaded with divalent nickel ions (Ni2+). The resin provides exceptional binding capacity of >50mg/ml of resin and performance that equals or exceeds that of Ni-NTA resins from other suppliers.
Ni-NTA resins are the most common choice for 6xHis-tag protein purifications by IMAC because of the four metal-binding sites on the chelate, which enable high-protein binding and low-metal ion leaching. The purity and yield of the target protein varies and is dependent on many factors including working conditions, size, conformation, expression levels, solubility and sample impurities.