Prometheus™ Ni-IDA Agarose Max Flow is a nickel-IMAC resin for large scale downstream purification of His-tagged proteins by Immobilized Metal Affinity Chromatography (IMAC). It is similar to Prometheus Ni-IDA Agarose, but is highly cross-linked and permits high flow rates, with good resolution in a minimal time frame. Ni-IDA Agarose Max Flow has high chemical and physical stability and was designed to meet the demands of industrial process separation.
The resin utilizes nickel (Ni2 ) for the purification of 6x histidine tagged proteins and binds to six histidine residues (6x His), a common tag used in protein purification. It consists of iminodiacetate (IDA - a tridentate chelating agent) coupled to 6% highly cross-linked agarose beads. The protein binding capacity is 5-10mg protein per ml resin, but the purity and yield of the target protein varies and is dependent on many factors including working conditions, size, conformation, expression levels, solubility and sample impurities.