Apex Brand Miller's LB Broth is used for propagation and maintenance of E. Coli. Dissolve 25 gm in 1 L distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes. Store dry at room temperature.
25g LB Broth Base/1L H20 = 10g tryptone, 5g yeast extract, 10g Sodium Chloride